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Fig. 3 | BMC Medicine

Fig. 3

From: Lycium barbarum polysaccharide improves dopamine metabolism and symptoms in an MPTP-induced model of Parkinson’s disease

Fig. 3

Evaluation of the therapeutic effect of LBP on the PD model. A–C Detection of the locomotor capacity of day 5 of the 5th instar larvae fed with LD-CD mixture (4 mM) or LBP (50, 100, and 200 mg/kg; n = 5/locomotor situation). D Determination of dopamine content in PD silkworm brains with LBP by UPLC (0, 50, and 100 mg/kg; n = 4/concentration). E Determination of BmTH activity in PD silkworm brains with LBP (0, 50, and 100 mg/kg) by enzyme-linked immunosorbent assay. F Detection of TH protein expression among untreated silkworm brains, PD silkworm brains, and brains of PD silkworms with LBP (50 mg/kg) by immunofluorescence. G, H Detection of the relative levels of BmDDC and BmPARK2 expression in brains of silkworms fed with LBP (0, 50, and 100mg/kg) by qPCR (n = 3/concentration). I, J Detection of total antioxidant capacity and MDA content in PD silkworms fed with LBP (0, 50, and 100 mg/kg; n = 3/concentration). All the experiments were performed at least three times and showed a similar pattern. Error bars depicted the mean with SD and were analysed using Student’s t-test. The “ns” is the abbreviation for “no significance”: *p < 0.05, **p < 0.01, ***p < 0.001

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