Table 1 Comparison of diagnostic performance of IHC biomarkers for detecting HSIL and HSIL+
Biomarker | Sensitivity (95% CI), % | p-valuea | Specificity (95% CI), % | p-valuea | PPV (95% CI), % | NPV (95% CI), % | AUC (95% CI) |
|---|---|---|---|---|---|---|---|
HSIL | |||||||
 p16INK4a | 92.6 (83.7–97.6) | Ref/0.248 | 63.4 (53.2–72.7) | Ref/<0.001 | 63.0 (52.8–72.4) | 92.8 (83.9–97.6) | 0.78 (0.72–0.84) |
 Ki-67 | 91.2 (81.8–96.7) | 1.000/0.480 | 80.2 (71.1–87.5) | 0.004/0.023 | 75.6 (64.9–84.4) | 93.1 (85.6–97.4) | 0.86 (0.81–0.91) |
 p16INK4a + Ki-67b | 88.2 (78.1–94.8) | 0.248/Ref | 87.1 (79.0–93.0) | <0.001/Ref | 82.2 (71.5–90.2) | 91.7 (84.2–96.3) | 0.88 (0.83–0.93) |
 TOP2A | 88.2 (78.1–94.8) | 0.450/1.000 | 87.1 (79.0–93.0) | <0.001/1.000 | 82.2 (71.5–90.2) | 91.7 (84.2–96.3) | 0.88 (0.83–0.93) |
 RFC4 | 88.2 (78.1–94.8) | 0.505/1.000 | 90.1 (82.5–95.1) | <0.001/0.579 | 85.7 (75.3–92.9) | 91.9 (84.7–96.4) | 0.89 (0.84–0.94) |
HSIL+ | |||||||
 p16INK4a | 95.3 (90.2–98.3) | Ref/0.041 | 63.4 (53.2–72.7) | Ref/<0.001 | 76.9 (69.6–83.2) | 91.4 (82.3–96.8) | 0.79 (0.74–0.84) |
 Ki-67 | 93.0 (87.2–96.8) | 0.505/0.248 | 80.2 (71.1–87.5) | 0.004/0.023 | 85.7 (78.8–91.1) | 90.0 (81.9–95.3) | 0.87 (0.82–0.91) |
 p16INK4a + Ki-67b | 90.7 (84.3–95.1) | 0.041/Ref | 87.1 (79.0–93.0) | <0.001/Ref | 90.0 (83.5–94.6) | 88.0 (80.0–93.6) | 0.89 (0.85–0.93) |
 TOP2A | 91.5 (85.3–95.7) | 0.228/1.000 | 87.1 (79.0–93.0) | <0.001/1.000 | 90.1 (83.6–94.6) | 88.9 (81.0–94.3) | 0.89 (0.85–0.93) |
 RFC4 | 91.5 (85.3–95.7) | 0.267/1.000 | 90.1 (82.5–95.1) | <0.001/0.579 | 92.2 (86.1–96.2) | 89.2 (81.5–94.5) | 0.91 (0.87–0.95) |