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Fig. 3 | BMC Medicine

Fig. 3

From: A novel 450-nm laser-mediated sinoporphyrin sodium-based photodynamic therapy induces autophagic cell death in gastric cancer through regulation of the ROS/PI3K/Akt/mTOR signaling pathway

Fig. 3

Effects of PDT on ROS production in GC cells. A, B Representative fluorescence images and quantitative analysis of intracellular ROS detection. Scale bar = 50 μm (n = 3, mean ± SD). C ESR spectra of TEMP/1O2 and DMPO/·OH after different treatments. D Flow cytometry analysis of the ROS amount after adding NAC (incubation 2 h). E Colony formation test of HGC27 cells after adding NAC (incubation 2 h). F EdU assay of HGC27 cells after adding NAC (incubation 2 h). Scale bar = 50 μm. *p < 0.05, **p < 0.01, and ***p < 0.001

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