Fig. 4

RNA-seq analysis indicated DEGs in the hippocampus were enriched in different synaptic pathways, and synapse-related genes were downregulated after prolonged anesthesia. A KEGG pathway enrichment bubble chart. B GO cellular component enrichment bubble chart. C GO molecular function enrichment bubble chart. D GO biological process enrichment bubble chart. From panels A to D, X-axis is the enrichment ratio (the ratio of the number of genes annotated to an entry in the selected gene set to the total number of genes annotated to the entry in the species), and Y-axis is KEGG pathway or GO term. The bubble size represents the number of genes annotated to the KEGG pathway or GO term. The color represents the enriched significance (n = 3 in each group). E–I Expression heat map of DEGs enriched in the synapse (E), cholinergic synapse (F), GABAergic synapse (G), dopaminergic synapse (H), and glutamatergic synapse (I). J–L Expression heat map of DEGs enriched in the postsynaptic density (J), postsynaptic membrane (K), and presynapse (L). From panels E to L, the horizontal axis is the z-score of the sample, and the vertical axis is the gene. The warmer the color block is, the higher the expression level is, and the colder the color block is, the lower the expression level is (n = 3 per group)