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Fig. 7 | BMC Medicine

Fig. 7

From: Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors

Fig. 7

Microglial depletion rescued cognitive dysfunction and ameliorated anxiety-like behaviors in SIN rats. A The schedule of the second experiment. We used chow with PLX3397 to feed rats for 21 days, depleting the microglial population. B iba1 (green) positive microglia were sharply depleted in the hippocampus after PLX3397 treatment. Scale bar = 200 μm. C PLX3397 treatment effectively eliminated microglial marker-iba1 expression in the hippocampus. D Statistical chart of iba1 expression between both groups (n = 4 per group). E, F Microglial depletion rescued cognitive dysfunction of SIN rats (n = 7 per group). In MWM, microglial depletion increased the number and time of entering the platform quadrant of SIN rats. G, H Microglial depletion ameliorated anxiety-like behaviors of SIN rats (n = 7 per group). In the OPF test, microglial depletion increased the time and number of entering the central zone of SIN rats. I, J Microglial depletion ameliorated anxiety-like behaviors of SIN rats (n = 7 per group). In the EPM test, microglial depletion increased the time and number of entering the opened arms of SIN rats. K, L Microglial depletion decreased the number of C1qa-colocalized SYP (n = 4 per group). Scale bar = 20 μm. Data was shown as Mean ± SD or median ± IQR, with *P < 0.05 or *P < 0.001; Sevo-AIN76A group vs. Sevo-PLX3397. Arrows represent colocalization

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