Fig. 8
RNA-seq analysis indicated DEGs in the hippocampus enriched in different synaptic pathways, and synapse-related genes were upregulated after microglial depletion. A Volcano map of DEGs. The X-axis represents the fold change of the difference after conversion to log2, and the Y-axis represents the significance value after conversion to −log10. The red represents 347 DEGs upregulated, the green represents 160 DEGs downregulated, and the gray represents non-DEGs. B KEGG pathway enrichment bubble chart. C GO cellular component enrichment bubble chart. D GO molecular function enrichment bubble chart. E GO biological process enrichment bubble chart. From panels B to E, X-axis is the enrichment ratio (the ratio of the number of genes annotated to an entry in the selected gene set to the total number of genes annotated to the entry in the species), and Y-axis is KEGG pathway or GO term. The size of the bubble represents the number of genes annotated to the KEGG pathway or GO term. The color represents the enriched significance (n = 3 in each group). F–K Expression heat map of DEGs enriched in the synapse (F), Schaffer collateral-CA1 synapse (G), glutamatergic synapse (H), cholinergic synapse (I), dopaminergic synapse (J), and GABAergic synapse (K). L–N Expression heat map of DEGs enriched in integral component of presynaptic membrane (L), integral component of postsynaptic density membrane (M), postsynaptic membrane (N). From panels F to N, the horizontal axis is the z-score of the sample, and the vertical axis is the gene. The warmer the color block is, the higher the expression level is, and the colder the color block is, the lower the expression level is (n = 3 per group)