Fig. 2

Freshly isolated mitochondria are more functional than frozen-thawed mitochondria. A Mitochondrial ATP contents in vehicle, frozen-thawed mitochondria, and fresh mitochondria immediately after mitochondrial isolation. One-way analysis of variance (ANOVA) with Šidák’s correction for post hoc comparisons was used. n = 3–4 per group. B Flow cytometry analysis of the mitochondrial membrane potential of the isolated mitochondria stained with JC-1 verified that the percentage of J-aggregates was markedly higher in the fresh-mito group than in the frozen-thawed-mito group. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) did not alter the ΔψM in the frozen-thawed-mito group. The one-way ANOVA with Šidák’s correction for post hoc comparisons was used. n = 4 per group