Fig. 8

Confocal fluorescence imaging reveals the persistence of the donor mitochondria within the organs at 1 and 24 h after cardiac arrest and resuscitation. Transplanted mitochondria (red) were observed in the brain, kidney, and spleen at 1 and 24 h after cardiac arrest. Arrowheads indicate the donated mitochondrial particles labeled using MitoTracker Deep Red dye before the injection. The cell nuclei were counterstained with DAPI (blue). Scale bar indicates 20 µm