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Fig. 2 | BMC Medicine

Fig. 2

From: Nitazoxanide inhibits acetylated KLF5-induced bone metastasis by modulating KLF5 function in prostate cancer

Fig. 2

Nitazoxanide exerts a preventive effect on Ac-KLF5-induced bone metastasis in prostate cancer cells. a Diagram of the experimental timeline. On day 0, cancer cells (PC-3-KQ-Luc or PC-3-KR-Luc) were injected into mice via the tail caudal artery, while nitazoxanide was delivered via intragastric administration. Bioluminescence intensities were measured at day 35. b, c Bioluminescence images (left) of mice carrying PC-3-KQ cells (b) or PC-3-KR cells (c) and receiving nitazoxanide (NTZ) or the solvent control (vehicle). Bioluminescence intensities are indicated by photon flux of the region of interest (ROI) (right panel, each dot represents a leg of a mouse). n = 12 legs/group. d Nitazoxanide treatment significantly reduced the bone tumor areas formed by PC-3-KQ cells while not affecting those of PC-3-KR cells. Femur sections were stained with H&E, and areas of tumor cells and entire bone sections were measured and compared between the NTZ treatment and the control group. Representative images are shown on the left, and statistical outputs are shown on the right. n = 6 tumors for each group. B, trabecular bone regions; BM, bone marrow regions; T, tumor regions. Scale bar, 100 μm. All data are presented as mean ± SD. **p < 0.01; ***, p < 0.001; ns, not significantly different; as estimated by student's t-test

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