Fig. 4
Small RNA sequencing analysis identifies miR-139-3p as an extracellular vesicle miRNA candidate. A, B Hierarchical clustering heat map (A) and volcano plot (B) showing 9 differentially expressed miRNAs between MSC-EV and MSCATV–EV. C Verification for the mRNA expressions in MSC-EV and MSCATV–EV by qPCR analysis, n = 3–4 per group. *P < 0.05, **P < 0.01, ***P < 0.001. D, E GO (D) and KEGG (E) enrichment analysis of target genes of three upregulated miRNAs (miR-139-3p, miR-320-3p, miR-501-3p) between MSC-EV and MSCATV–EV. F Interaction network between target genes of three upregulated miRNAs (miR-139-3p, miR-320-3p, miR-501-3p) and upregulated genes in patients suffered from first acute myocardial infarction (GEO: GSE24591). G Interaction network between target genes of three upregulated miRNAs (miR-139-3p, miR-320-3p, miR-501-3p) and downregulated genes in human monocyte-derived M2 macrophages (GEO: GSE32164). H The intersection between the three upregulated miRNAs of miR-139-3p, miR-320-3p, miR-501-3p, and 25 downregulated miRNAs in patients suffered from first acute myocardial infarction (GEO: GSE24591) is miR-139-3p. ATV, atorvastatin; EV, extracellular vesicle; GEO, gene expression omnibus; GO, gene ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; MSCs, mesenchymal stem cells; miRNA, micro-RNA; qPCR, quantitative polymerase chain reaction