Fig. 1

ACT1 expression in human CRC tissue and correlation with TAMs. (A) ACT1 expression pattern in healthy intestinal tissue (n = 51) and tumor tissue (n = 623) of colorectal adenocarcinoma patients analyzed from TCGA data. (B) ACT1 expression pattern in tumor tissue of distinct stages of colorectal adenocarcinoma patients analyzed from TCGA data (Normal: n = 51; Stage I: n = 111; Stage II: n = 238; Stage III: n = 184; Stage IV: n = 90). (C) The correlation of macrophage infiltration with ACT1 expression in colorectal adenocarcinoma patients analyzed from TISIDB data, n = 626. (D) Representative immunohistochemistry staining of ACT1 and CD68⁺ macrophage in the paired peri-tumor or tumor tissues of colorectal adenocarcinoma patients (n = 65). Peri-tumor tissues (black rectangle) and tumors (red rectangle) (a). Statistical assay of ACT1 (b) and CD68⁺ macrophages (c), respectively. Scale bar in (a), 200 μm (upper) and 50 μm (lower). (E) ACT1 colocalization with CD68⁺ macrophages in the peritumor and tumor tissues of CRC patients. Scale bar, 50 μm. (F) Number of CD68⁺ cells in ACT1-low expression tumor or ACT1-high tumor of CRC patients. (G) Immunohistochemical staining of E-cadherin and snail. Scale bar, 50 μm (a). Statistical analysis of relative expression of E-cadherin (b) and snail (c) in tumor enriched CD68⁺ACT1^high macrophages or CD68⁺ACT1.^low macrophages of CRC patients. Significant difference between the groups, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (Student t test)