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Fig. 5 | BMC Medicine

Fig. 5

From: NF-κB Activator 1 downregulation in macrophages activates STAT3 to promote adenoma-adenocarcinoma transition and immunosuppression in colorectal cancer

Fig. 5

Macrophage-specific Act1-knockdown increased CXCLs secretion and PD-L1 expression via STAT3 activation. A. (a) Schematic representation of the experimental design. (b) The effect of STAT3 silencing in anti-Act1 macrophages Representative western blot images of pSTAT3, p-p65, CXCL10, and PD-L1 expression and quantification in anti-Act1 macrophages cocultured with MC38 cells. (B) The effect of STAT3 silencing in anti-Act1 macrophages on migration after coculture with MC38 cells for 24 h. Scale bar, 50 μm. (C) Representative western blot images and quantification of epithelial-mesenchymal transition markers in MC38 cells cocultured with macrophages, n = 3. Significant difference between the groups, *p < 0.05, #p < 0.05, ** p < 0.01, ##p < 0.01, ***p < 0.001, and ****p < 0.0001. GAPDH or β-actin was used as an internal loading control

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