Fig. 3

Impact of Endotoxin (LPS) on Human Primary Adipocyte Browning. Lean (A1/B1/C1/D1/E1/F1) and obese (A2/B2/C2/D2/E2/F2) primary human adipocytes were differentiated with/without 2 µM rosiglitazone (Rosi), 100 ng/mL lipopolysaccharide (LPS100) or a combination of the two. Browning genes uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α), cell death-inducing DFFA-like effector A (CIDEA), ELOVL fatty acid elongase 3 (ELOVL3), perilipin 5 (PLIN5) and Solute Carrier Family 27 Member 2 (SLC27A2) were analyzed using qRT-PCR with L19 as a housekeeping control. Data represent mean ± standard error of the mean (SEM). The two-way ANOVA test was used to test significance levels; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to control; † p < 0.05, †† p < 0.01, ††† p < 0.001, †††† p < 0.0001 compared to Rosi treatment; × p < 0.05, ×  ×  ×  × p < 0.0001 compared to LPS treatment