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Fig. 5 | BMC Medicine

Fig. 5

From: The impact of metabolic endotoxaemia on the browning process in human adipocytes

Fig. 5

Impact of Endotoxin (LPS) on Mitochondrial Function. Primary human adipocytes were differentiated in the presence of 2 µM rosiglitazone (Rosi), 100 ng/mL lipopolysaccharide (LPS) or a combination of the two. A Following differentiation, isoproterenol was injected (to a final concentration of 10 µM) whilst oxygen consumption rate (OCR) was measured. B A Seahorse Mito Stress Test was also performed on the differentiated cells to assess key parameters of mitochondrial respiration. Dotted lines indicate injections into media of the specific compounds: isoproterenol, oligomycin, carbonyl cyanite-4 (trifluoromethoxy) phenylhydrazone (FCCP) and rotenone/antimycin A (R&A). C UCP1 protein expression was assessed in differentiated cells via Western blot. One-way ANOVA was carried out to assess significant differences. Seahorse data: * p < 0.05 control vs. LPS; # p < 0.05, ## p < 0.01 LPS vs. Rosi; x p < 0.05 LPS vs. Rosi + LPS; f Rosi vs. Rosi + LPS; † control vs. Rosi. UCP1 data: + p < 0.05 compared to control; ^^^ p < 0.001 compared to Rosi

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