Fig. 6

Impact of Endotoxin (LPS) on Mitochondrial Dynamics. Following differentiation of lean (A1, B1, C1, D1, n = 4) and obese (A2, B2, C2, D2, n = 3) primary human adipocytes with 2 µM rosiglitazone (Rosi), 100 ng/mL lipopolysaccharide (LPS100) or a combination of the two, mitochondrial fission genes dynamin-related protein 1 (DRP1) and mitochondrial fission 1 (FIS1), as well as fusion genes mitofusin 2 (MFN2) and mitochondrial dynamin like GTPase (OPA1) were analyzed via RT-PCR, with L19 as a housekeeping control. Data represent mean ± SEM. The two-way ANOVA test was used to test significance; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to control, † p < 0.05, †† p < 0.01, ††† p < 0.001, †††† p < 0.0001 compared to Rosi treatment, x p < 0.05, xx p < 0.01, xxx p < 0.001, xxxxp < 0.0001 compared to LPS