Fig. 8From: Single-cell sequencing reveals the immune microenvironment landscape related to anti-PD-1 resistance in metastatic colorectal cancer with high microsatellite instabilityCorrelation between IL-1β and MMP9 in the mouse xenograft model. qPCR of (A) IL-1β and (B) MMP9 mRNA expression in each treatment group (n = 3). C Western blotting analysis (n = 3). Grayscale analysis of (D) IL-1β and (E) MMP9 protein (n = 3). IHC staining and scores of (F, G) IL-1β and (H, I) MMP9 expression (n = 5). (J) Pearson correlation analysis of MMP9 and IL-1β expression (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001Back to article page