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Fig. 5 | BMC Medicine

Fig. 5

From: Adipsin alleviates cardiac microvascular injury in diabetic cardiomyopathy through Csk-dependent signaling mechanism

Fig. 5

Adipsin promotes proliferation and suppresses apoptosis in CMECs under HG + PA conditions. A Representative images of apoptosis evaluated by TUNEL Assay (green cells) in vitro. Scale bar = 100 μm. B Representative dot plots displaying proportions of apoptotic cells. D Quantitative analysis of Annexin V-FITC-positive cells. C The apoptotic index was expressed as the number of TUNEL-positive cells normalized to the total number of cells. E Representative immunofluorescence images of CMEC proliferation evaluated by EdU-based proliferation assay. Scale bar = 100 μm. F Quantitative analysis of EdU-positive cells. G Cell viability determined using CCK-8 assay. Data were presented as mean ± SEM. One-way ANOVA was used for statistical analysis

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