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Fig. 6 | BMC Medicine

Fig. 6

From: Adipsin alleviates cardiac microvascular injury in diabetic cardiomyopathy through Csk-dependent signaling mechanism

Fig. 6

Adipsin promotes CMECs migration and tube formation while inhibits hyperpermeability in CMECs treated with HG + PA. A Wound healing scratches were imaged at 12 h after the initial scratch time point. Black lines indicated the boundary of the scratch. Scale bar = 500 μm. B Migration distances from edges of the wound. C Representative images of migrated cells in the transwell migration assay. Scale bar = 200 μm. D Quantitative analysis of migrated cells. E Representative images of tube formation assay. Scale bar = 200 μm. F Tube formation assays were quantified by measuring tube length. G Quantitative analysis of FITC-conjugated Dextran intensity evaluating monolayer cellular permeability. H Measurements of trans-endothelial electrical resistance (TEER). I Representative immunofluorescence images of cell-cell junctions, and white arrowheads indicated endothelial cell junctions. Scale bar = 50 μm. Data were presented as mean ± SEM. One-way ANOVA was used for statistical analysis

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