Fig. 7
Adipsin interrupts HG + PA-induced VE-cadherin internalization by inhibiting Src activity. A Representative images of immunofluorescence staining for Adipsin in CMECs treated with AdipsinLSL/LSL&Exosomes or AdipsinLSL/LSL-Cre&Exosomes isolated from adipose tissues. Scale bar = 50 μm. B Quantitative analysis of Adipsin intensity in A. C Representative Western blot images for VE-cadherin (total), phospho-VE-cadherin (Tyr731), and phospho-VE-cadherin (Tyr685). D–F Quantitative analysis of Western blot images from three independent experiments in C. G Representative Western blot images for VE-cadherin (total), VE-cadherin (membrane), phospho-VE-cadherin (Tyr731), phospho-VE-cadherin (Tyr685), Src, and phospho-Src (Tyr416). H–I Quantitative Western blot analysis from three independent experiments in G. Data were presented as mean ± SEM. For B, H, and I, Student’s t‐test was used for statistical analysis. For D–F, one-way ANOVA was used for statistical analysis