Fig. 5
miR-CVB3 + CpGMel suppresses tumor growth in B16F10 tumor-bearing mice. A IHC staining of CAR in B16F10 tumor (representative of 3 slides). Scale bar = 100 μm. B The tumor size after different treatments (n = 3 mice/group). C, D Average tumor weights (C) and percentage of tumor suppression rate (D) in different groups (n = 3 mice/group). E IHC staining of VP1 in B16F10 tumor collected at day 14 post-treatment (representative of 2–3 slides/treatment). Scale bar = 100 μm. F H&E staining of different tissues collected at day 14 post-treatment (representative of 3 slides/treatment). Scale bar = 50 μm. G Pathological scores of the H&E staining (n = 3 mice/group). The concentrations of each agent for animal treatment were as follows: miR-CVB3 at 10.5 pfu/mouse, CpG at 50 μg/mouse, and melittin at 100 μg/mouse. Data in this figure were analyzed by one-way ANOVA followed by Tukey’s test to determine differences (*, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001)