New tuberculosis vaccines in India: modelling the potential health and economic impacts of adolescent/adult vaccination with M72/AS01E and BCG-revaccination

Background India had an estimated 2.9 million tuberculosis cases and 506 thousand deaths in 2021. Novel vaccines effective in adolescents and adults could reduce this burden. M72/AS01E and BCG-revaccination have recently completed phase IIb trials and estimates of their population-level impact are needed. We estimated the potential health and economic impact of M72/AS01E and BCG-revaccination in India and investigated the impact of variation in vaccine characteristics and delivery strategies. Methods We developed an age-stratified compartmental tuberculosis transmission model for India calibrated to country-specific epidemiology. We projected baseline epidemiology to 2050 assuming no-new-vaccine introduction, and M72/AS01E and BCG-revaccination scenarios over 2025–2050 exploring uncertainty in product characteristics (vaccine efficacy, mechanism of effect, infection status required for vaccine efficacy, duration of protection) and implementation (achieved vaccine coverage and ages targeted). We estimated reductions in tuberculosis cases and deaths by each scenario compared to the no-new-vaccine baseline, as well as costs and cost-effectiveness from health-system and societal perspectives. Results M72/AS01E scenarios were predicted to avert 40% more tuberculosis cases and deaths by 2050 compared to BCG-revaccination scenarios. Cost-effectiveness ratios for M72/AS01E vaccines were around seven times higher than BCG-revaccination, but nearly all scenarios were cost-effective. The estimated average incremental cost was US$190 million for M72/AS01E and US$23 million for BCG-revaccination per year. Sources of uncertainty included whether M72/AS01E was efficacious in uninfected individuals at vaccination, and if BCG-revaccination could prevent disease. Conclusions M72/AS01E and BCG-revaccination could be impactful and cost-effective in India. However, there is great uncertainty in impact, especially given the unknowns surrounding the mechanism of effect and infection status required for vaccine efficacy. Greater investment in vaccine development and delivery is needed to resolve these unknowns in vaccine product characteristics. Supplementary Information The online version contains supplementary material available at 10.1186/s12916-023-02992-7.


Background
India has the largest global burden of tuberculosis.In 2021, there were an estimated 2.9 million cases and 506 thousand deaths-representing approximately 30% of the total globally [1].The COVID-19 pandemic has negatively impacted tuberculosis prevention and care in India, with increases in the number of deaths per year seen for the first time since 2007 [1,2].Delays in diagnosis and treatment due to surveillance systems impacted by the pandemic (over 30% fewer notifications reported in 2021 than 2019) may lead to increases in the disease burden [1,2].
Tuberculosis is a key focus for the Indian government.The National Strategic Plan to End Tuberculosis in India 2020-2025, developed by the National Tuberculosis Elimination Programme (NTEP), outlines ambitious goals for reducing Mycobacterium tuberculosis (Mtb) transmission, preventing tuberculosis disease, and addressing social determinants of health [3].Despite the COVID-19 pandemic, the NTEP has made progress toward these goals, including expanding molecular diagnostics, implementing tuberculosis-COVID bidirectional screening, and expanding policy on preventive therapy to include all household contacts of people diagnosed with pulmonary tuberculosis [4].
The National Strategic Plan also calls for further development in tuberculosis vaccines, which has been a high priority for global organisations such as the World Health Organization (WHO).A recently completed WHO-commissioned study assessing the full value of tuberculosis vaccines made a strong case from the health and economic perspectives for continued investment [5][6][7][8][9], and previous work has demonstrated that novel vaccines or vaccination strategies will be needed to eliminate tuberculosis [10,11].
Currently, sixteen candidates are in various phases throughout the vaccine pipeline, being trialled in a variety of ages and spanning prevention of disease, infection, and recurrence endpoints [12].A phase IIb trial of M72/ AS01 E in adolescents and adults infected with Mtb demonstrated a prevention of disease efficacy of 49.7% (95% confidence interval: 2.1-74.2) after 3 years of follow-up [13].However, M72/AS01 E would need a supportive phase III trial for licensure, which is planned but likely to require years before results are available to inform policy.
Revaccination of uninfected adolescents with the Bacillus Calmette-Guérin (BCG) vaccine was assessed as a third parallel arm in a separate phase IIb trial and demonstrated an efficacy of 45.4% (6.4-68.1)against sustained infection [14], and an additional phase IIb confirmation trial is underway to verify this finding, with results expected mid-2024 [15].The original Chingleput BCG vaccination trial reported efficacy of 27% (− 8 to 50) against disease in children and no efficacy in adults [16].A re-analysis of trial data restricted to participants with prior BCG vaccination and no tuberculosis disease at the time of vaccination showed a protective efficacy of 36%  against disease [17].As BCG is already licensed, introducing BCG-revaccination may only require a policy change, which could happen quickly.
India is arguably the most important country for global tuberculosis elimination, and policy-makers require country-specific evidence of the anticipated health, cost, and budget impacts of specific vaccine candidates.As vaccines enter phase III trials, it is important to predict how variation in vaccine profile and implementation will affect the impact to maximise benefits and reduce delays between licensure and delivery.We estimated the potential health and economic impact of M72/AS01 E and BCG-revaccination in India and investigated the impact of variation in vaccine characteristics and delivery strategies.

Data
We obtained demographic data for India from the United Nations Population Division with estimates for single ages and years from 1900 to 2100 [18].Tuberculosis disease and infection prevalence estimates were derived from the National TB Prevalence Survey in India 2019-2021 [19].Incidence, notifications, and mortality estimates were obtained from WHO [2].

Structure
We adapted previous models and developed a compartmental dynamic model of tuberculosis in India [5,11,20].Our model was stratified by tuberculosis natural history and treatment, differences in access-to-care, vaccination, and age.We represented tuberculosis natural history by allowing for Mtb infection along a spectrum from uninfected to active clinical disease.We assumed a progressive loss of ability to reactivate following infection, with a monotonic decline in reactivation rates for subsequent latency compartments.Active disease was represented by both subclinical and clinical tuberculosis compartments to align with prevalence survey data [19].Antituberculosis treatment was assumed to begin in 1960 and increase following a sigmoid curve to 2020.Due to the large contribution of private sector treatment in India, we incorporated differences in treatment mortality and completion probabilities between the public and private sectors.Full model structure and parameters are in Additional file 1 Sects.1, 2 [5,18,[21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37].

No-new-vaccine baselines
Assuming the quality and coverage of services remain constant post-2020, we used the calibrated model to project baseline epidemiology to 2050 (the Status Quo nonew-vaccine baseline).We assumed that neonatal BCG vaccination would not be discontinued during the period of our analysis and was not explicitly modelled as its effect is implicitly included in country burden estimates.
As an alternative future scenario, we calibrated a Strengthened Current Interventions no-new-vaccine baseline.This baseline assumed scale-up of non-vaccine tuberculosis interventions between 2021 and 2035 to meet the target of a 50% reduction in tuberculosis incidence in 2035 compared to the 2015 estimates.This scale-up was included in the model by introducing multipliers on the rate of progression to disease and in the force of infection equation.

Vaccine scenarios
Using the calibrated Status Quono-new-vaccine model, we simulated Basecase scenarios over 2025-2050 for each product with characteristics informed a priori by clinical trial data and expert opinion [13,14].The Basecase M72/AS01 E scenario assumed a 50% efficacy prevention of disease vaccine with 10-year protection, efficacious with any infection status aside from active disease at vaccination.We assumed the vaccine would be introduced in 2030 routinely to those aged 15 (reaching 80% coverage) and as a campaign for ages 16-34 (reaching 70% coverage), with a repeat campaign in 2040.Based on expert advice, the vaccine price was $2.50 per dose, assuming two doses per course.
The Basecase BCG-revaccination scenario assumed a 45% efficacy vaccine to prevent infection with 10-year protection, and efficacious without infection at time of vaccination.We assumed the vaccine would be introduced in 2025 routinely to those aged 10 (reaching 80% coverage) and as a campaign for ages 11-18 (reaching 80% coverage) with repeat campaigns in 2035 and 2045.Based on the average estimated BCG price from UNICEF [53], the vaccine price was set at US$0.17 per dose, assuming one dose per course.
Vaccine introduction costs for both vaccine products were assumed to be US$2.40(95% uncertainty interval = 1.20-4.80)per individual in the targeted age group based on vaccine introduction support policy from Gavi, the Vaccine Alliance [54].A further US$0.11(0.06-0.22) supply costs and US$2.50 (1.00-5.00)delivery costs per dose were included [55], as well as US$0.94 (0.13-1.52) in patient and caregiver productivity losses per dose, to account for the time taken to receive vaccination [56,57].We assumed a 5% wastage rate.
Through consultation with vaccine and country-specific experts, we established specific M72/AS01 E and BCG-revaccination Policy Scenarios and Vaccine Characteristic and Coverage Scenarios.Policy Scenarios represented features of vaccination strategy under the control of decision-makers, which compared different age groups to target for vaccination.Vaccine Characteristic and Coverage Scenarios represented current uncertainties around vaccine performance and uptake, in which we varied unknowns in vaccine profile (such as efficacy, duration of protection, mechanism of effect) and achieved coverage, univariately from each Basecase scenario.We compared Policy Scenarios to identify the optimal implementation approach, and Vaccine Characteristic and Coverage Scenarios to quantify the impact of different sources of uncertainty (Table 1).Further details are provided in Additional file 1 Sect.4 [13,14,58].

Outcomes
We estimated the cumulative number of tuberculosis cases and deaths averted between vaccine introduction and 2050 for each scenario compared to the predicted numbers in both no-new-vaccine baselines.
For each vaccine product, we conducted cost-effectiveness analyses for the Policy Scenarios indicated in Table 1, discounting both costs and health outcomes to 2025 (when vaccination began) at 3% per year as per guidelines [59].We calculated the difference in total disability-adjusted life years (DALYs) from vaccine introduction to 2050, using the disability weight for tuberculosis disease from the Global Burden of Disease 2019 study [60], and country-and age-specific life expectancy estimates from the United Nations Development Programme assuming no post-tuberculosis morbidity or mortality [61].We calculated incremental cost-effectiveness ratios (ICERs) as the ratio of mean incremental costs to mean incremental benefits in DALYs averted, and 95% uncertainty intervals from the health-system perspective for each efficient strategy for the analytic period 2025-2050.Higher cost-effectiveness ratios indicate greater spending is needed to achieve health improvements, such that the intervention is less likely to be cost-effective.We measured cost-effectiveness by 2050 against three India-specific cost thresholds: 1 × gross domestic product (GDP) per capita (US$1,927.71)[57], and country-level opportunity cost thresholds defined by Ochalek et al. (country-level upper [US$363] and lower [US$264] bounds) [62].
To investigate how the consequences of vaccine introduction (versus no vaccination) changed based on the vaccine product characteristics, we examined the difference in ICERs for Vaccine Characteristic and Coverage Scenarios compared to the no-new-vaccine baseline assuming the vaccine was introduced using the delivery strategy from the most efficient Policy Scenario at the country-level lower bound.

Results
The Status Quo baseline model fits all 19 calibration targets with at least 1000 parameter sets.Epidemiological projections from 2020 to 2050 are in Additional file 1 Sect.7. The Status Quo baseline predicted 72.2 (63.3-79.7) million incident tuberculosis cases and 13.8 (12.9-15.2) million tuberculosis deaths between 2025 and 2050.Assuming current non-vaccine tuberculosis interventions would be strengthened such that the incidence rate in 2035 was 50% of the incidence rate in 2015, the Strengthened Current Interventions baseline predicted 36.0 (28.9-66.4) million incident cases and 7.6 (6.1-13.2) million deaths between 2025 and 2050.
With the Status Quo no-new-vaccine baseline, we found a 50% efficacy M72/AS01 E prevention of disease vaccine, efficacious with any infection status, introduced in 2030 routinely to 15-year-olds and as a campaign for ages 16-34 (the Basecase M72/AS01 E scenario), could avert approximately 12.7 (11.0-14.6) million cases and 2.0 (1.8-2.4) million deaths between 2030 and 2050 (Fig. 1).With a 70% efficacy vaccine, the number of averted cases and deaths by 2050 could be increased by 32-35% but delaying introduction of a vaccine until 2036 could lead to 5.2 million more cases and 968 thousand more deaths compared to the Basecase M72/AS01 E scenario before 2050 (Fig. 1).If the vaccine was only efficacious with current infection at vaccination, 5.8 million fewer cases and 900 thousand fewer deaths could be averted compared to the Basecase M72/AS01 E scenario.
A 45% efficacy prevention of infection BCG vaccine, efficacious in those with no current infection, introduced in 2025 as routine vaccination of 10-year-olds and a campaign for ages 11-18 (the Basecase BCG-revaccination scenario) could avert 9.0 (7.8-10.4) million cases and 1.5 (1.3-1.8) million deaths (Fig. 1).If the vaccine prevented infection and disease, 3.4 million more cases and 600 thousand more deaths could be averted by 2050 compared to the Basecase BCG-revaccination scenario.Fewer numbers could be averted compared to the Basecase BCG-revaccination scenario with reduced duration of protection, later introduction, lower coverage, or only delivering the vaccine to ages 60 years and older (Fig. 1).
Comparing the two products, even with a later introduction year for M72/AS01 E scenarios, we found a higher health impact from M72/AS01 E vaccines compared to BCG-revaccination.The Basecase M72/AS01 E scenario was predicted to avert around 40% more tuberculosis cases and deaths before 2050 than the Basecase BCGrevaccination scenario.
With the Strengthened Current Interventions baseline, the Basecase M72/AS01 E scenario could avert 3.0 (1.1-11.3) million tuberculosis cases and 0.51 (0.19-1.9) million tuberculosis deaths between 2025 and 2050, averting 8.3% of the median total cases and 6.7% of the median total deaths predicted to occur during the same period.The Basecase BCG-revaccination scenario could avert 1.9 (0.42-8.0) million cases and 0.34 (0.08-1.4) million deaths between 2025 and 2050, or 5.3% of the median total tuberculosis cases and 4.5% of the median total tuberculosis deaths predicted to occur during the same period.Health impact values for all scenarios of both vaccines are in Additional file 1 Sect.8. Cost-effectiveness analysis is shown in Table 2 and Fig. 2 for the Policy Scenarios for each vaccine product.For M72/AS01 E , delivering the vaccine routinely to those age 60 and as a campaign for ages 61 + (Elderly Ages M72/ AS01 E scenario) was not efficient and removed from consideration.Scenarios delivering the vaccine routinely to age 15 and as a campaign for ages 16-34 (Basecase M72/ AS01 E scenario) and delivering the vaccine as a campaign for ages 18-55 (Older Ages M72/AS01 E scenario) were considered efficient and displayed on the efficiency frontier in Fig. 2. The Basecase M72/AS01 E scenario was optimal at both country-level thresholds (ICER = US$145 per DALY averted), and the Older Ages M72/AS01 E scenario was optimal at 1 × GDP threshold (ICER = US$1,120 per DALY averted).The incremental cost of the Basecase M72/AS01 E scenario was US$5.3 billion, with vaccination averting 36.9 million of the 4.0 billion DALYs predicted by the no-new-vaccine baseline between 2025 and 2050.
For BCG-revaccination, delivering the vaccine routinely to those age 60 and as a campaign for ages 61 + (Elderly Ages BCG-revaccination scenario) was dominated by other strategies and removed from  Figure 3 displays the ICERs for each Vaccine Characteristic and Coverage Scenario compared to the no-newvaccine baseline for each vaccine product.For every M72/AS01 E scenario shown in the figure, we assumed that the vaccine would be introduced routinely to those aged 15 and as a campaign to ages 16-34 (the most efficient strategy at the country-level lower bound from the cost-effectiveness analysis).Even with changes in the vaccine product characteristics, introducing an M72/ AS01 E vaccine would be cost-effective compared to not implementing a vaccine (Fig. 3).For every BCG-revaccination scenario, we assumed that the vaccine would be introduced routinely to those aged 10 and as a campaign to ages 11-18 (the most efficient strategy at the countrylevel lower bound from the cost-effectiveness analysis).Similarly, regardless of the resulting product characteristics, introducing BCG-revaccination to this age group would be cost-effective compared to not implementing a vaccine (Fig. 3).
From the health-system perspective, the annual average cost of vaccination in the Basecase M72/AS01 E scenario was approximately US$251   Figure 4 demonstrates the distribution of costs and cost-savings per year from vaccine introduction to 2050

Discussion
We found that M72/AS01 E scenarios could avert approximately 12.7 (11.0-14.6) million cases and 2.0 (1.8-2.4) million deaths, and BCG-revaccination scenarios could avert approximately 9.0 (7.8-10.4) million cases and 1.5 (1.3-1.8) million deaths of the 72.2 (63.3-79.7) million cases and 13.8 (6.1-13.2) million deaths predicted by the Status Quo baseline between 2025 and 2050.Costeffectiveness ratios for the Basecase M72/AS01 E scenario were around seven times higher than that for the Basecase BCG-revaccination scenario, but regardless of the realised product characteristics, nearly all Vaccine Characteristic and Coverage Scenarios were cost-effective at the most conservative country-level threshold compared to the no-new-vaccine baseline.The average annual cost of M72/AS01 E vaccination was four times greater than BCG-revaccination.Introducing the vaccine could lead to an annual incremental programme cost of US$190 million for M72/AS01 E and US$23 million for BCGrevaccination, accounting for vaccination costs as well as savings in diagnostic and treatment costs.
Our modelling demonstrated a 40% greater health impact from M72/AS01 E compared to BCG-revaccination.The difference in impact was due to assumptions made on vaccine characteristics and delivery.Based on clinical trial data and expert opinion, we assumed the Basecase M72/ AS01 E vaccine would prevent disease and be efficacious in everyone without active disease at vaccination.In contrast, based on trial data [14,70], we assumed the Basecase BCG-revaccination scenario would be efficacious only in people without infection at the time of vaccination, and would prevent infection.Therefore, M72/AS01 E would be effective in a larger proportion of the population compared to BCG-revaccination and have a more rapid impact on tuberculosis incidence.The effect of BCG-revaccination on disease will be delayed by the time between vaccination and infection in addition to the time from infection to disease.This is consistent with previous work showing more rapid impact on disease of a vaccine that prevents disease directly in those currently infected [11].
As demonstrated in the National Tuberculosis Prevalence Survey, the highest tuberculosis prevalence estimates are found in older adolescents and adults [19].The Basecase scenario for M72/AS01 E delivered the vaccine routinely to those aged 15 and as a campaign for ages 16-34, as opposed to the Basecase BCG-revaccination scenario which was targeted routinely to those aged 10 and a campaign for ages 11-18.As the M72/AS01 E vaccine was targeted to an age group with a higher burden, we saw an increased impact on the burden.
We explored variation in decisions regarding delivery and the realised vaccine by evaluating Policy Scenarios and Vaccine Characteristic and Coverage Scenarios where we varied characteristics univariately from the Basecase for each vaccine product, and found all uncertainties had the anticipated direction of effect.Both M72/AS01 E and BCG-revaccination were highly influenced by vaccine efficacy and duration of protection, with higher efficacies and longer durations of protection increasing health impact and cost-effectiveness.Key sources of uncertainty were whether M72/AS01 E was efficacious without infection vaccination, and if BCG-revaccination was also able to prevent disease in adults, both of which are key areas of research.Given the uncertainty surrounding prevention of disease efficacy from BCG-revaccination, any roll out of BCG to adolescents and adults should be rigorously evaluated with a prevention of disease outcome.
M72/AS01 E scenarios were predicted to have higher vaccination costs per year compared to BCG-revaccination.The assumed M72/AS01 E vaccine price per course of US$5.00 (two doses for US$2.50 each) was almost 30 times the US$0.17 price per course of BCG-revaccination, in addition to duplicated delivery and supply costs necessary to deliver two doses of M72/AS01 E compared to one dose of BCG.These cost differences directly contribute to higher cost-effectiveness ratios and larger annual cost for M72/AS01 E .Our analyses demonstrated that both vaccines could be cost-effective, aligning with previous cost-effectiveness analyses of tuberculosis vaccines [6,32].While vaccination could have a substantial budget impact, costs could be partially offset with diagnostic and treatment savings.
Comparing the ICERs for Vaccine Characteristic and Coverage Scenarios, we see that even if the product characteristics change from the Basecase scenario for each vaccine product, the decision remains the same.Introducing M72/AS01 E or BCG-revaccination would be a cost-effective intervention.
This work has limitations.We modelled the impact of specific M72/AS01 E and BCG-revaccination scenarios with characteristics based on clinical trial data and consultation with vaccine and country-specific experts, but it will be many years before the actual characteristics are known.To capture some uncertainty, we univariately varied efficacy, duration of protection, whether the vaccine prevents only infection or disease or both, and who the vaccine would be efficacious in.The majority of scenarios continued to demonstrate large potential health impact and cost-effectiveness.We were not investigating the separate question of determining the range of plausible conditions that M72/AS01 E would no longer be cost-effective or scenarios where BCGrevaccination would have a greater impact, which is an important area for future work to address.
The Basecase M72/AS01 E scenario assumed efficacy with any infection status at vaccination, implying that the vaccine would work in both those who were infected with Mtb and those who were uninfected.While the Phase IIb trial of M72/AS01 E only enrolled adults with a positive interferon-gamma release assay (IGRA) value, previous trials have indicated that an immune response is invoked in adolescents both with and without infection, and the phase III trial will enrol IGRA positive and negative individuals aged 15-44 years.Therefore, the expected initial indicated population is everyone within these ages, and thus we aligned our primary assumption for host infection status with this.We evaluated a scenario assuming only current infection at vaccination and determined that efficacy in those who are uninfected at the time of vaccination is important to maximise health impact and cost-effectiveness.Investigating whether M72/AS01 E works in populations with any infection status is a key aspect for future research.
We modelled a small subset of age-targeted delivery scenarios, which may differ from the strategies India will choose.We evaluated alternatives informed by expert opinion and results from interviews with key decisionmakers in India [58], but did not investigate targeting specific groups, such as healthcare workers, people completing tuberculosis treatment, or household contacts of people with tuberculosis, who could be at high risk of developing tuberculosis disease and may be prioritised for vaccination.This strategy has previously been suggested to have a high population-level impact per individual vaccinated [71-73 and greater than 45% for BCG-revaccination (aligning with the estimates of protection from the Phase IIb trials).However, the true vaccine efficacy is currently unknown, and if our assumptions were too optimistic, we may have overestimated the health and economic impacts.
The burden of tuberculosis varies widely across India.From the recent National Tuberculosis Prevalence Survey, the prevalence per 100,000 population of pulmonary tuberculosis among adults ranged from 115  in Kerala to 534 (365-704) in Delhi [19].Optimal delivery strategies may vary by state, given the vast differences in age composition, population size, and tuberculosis burden.Modelling specific regions to investigate the generalisability of national predictions is an important area of future research.
We ran cost-effectiveness analysis for each product on the age-targeting Policy Scenarios.We selected the Basecase vaccine profile characteristics for each vaccine product as it incorporates the primary assumptions from experts in the field on the likely vaccine product characteristics, but we did not run cost-effective analysis for the age-targeting strategies with other vaccine characteristics.
Our work is a modelling exercise, and limitations associated with mathematical models apply.We developed our tuberculosis natural history structure incorporating recent advances in knowledge regarding the clinical course of disease, such as subclinical tuberculosis and a latency structure with a progressive loss in the to reactivate.If our assumptions around these novel aspects, particularly around interactions with vaccines, are incorrect, we may have over-or underestimated the impact.While we used the best available data to inform calibration targets and natural history parameters, we were limited by what was available.We ensured that the modelled trends aligned with the most recent estimates of tuberculosis burden, as vaccines are not anticipated to be introduced until at least 2025.However, with only one estimate of whole-country disease prevalence and one estimate of whole-county infection prevalence in India, we were restricted with what we could infer about these measures over time, which highlights the need for more regularly collected data on disease prevalence and infection.We made decisions on natural history parameter ranges based on the most recent literature available, but this still resulted in wide prior ranges for some parameters.Further data collection into these areas would improve model estimates.
We projected the no-new-vaccine baseline as Status Quo, where we assume that the rate and quality of services remained constant from 2020 onwards, and the resulting trends in burden from 2020-2050 follows a slight decline.Given the commitment of the Indian government to improvements in tuberculosis care, prevention, and ending the tuberculosis epidemic, our model could be overestimating the burden of tuberculosis.Therefore, our health and economic impacts may be overestimated.We ran a sensitivity analysis for the Basecase scenario for each vaccine product using the Strengthened Current Interventions no-new-vaccine baseline.We found that vaccines would still have a positive health impact and would be costeffective even if the incidence rate was declining faster than assumed in our primary scenario.We demonstrated that vaccines could also be an impactful and cost-effective investment for the Indian government if future tuberculosis burden is much lower.
The results from this study could be used to inform policy-makers considering novel tuberculosis vaccine introduction.We have demonstrated that both BCGrevaccination and M72/AS01 E could have a positive health impact and would be cost-effective if delivered, given our current assumptions.We evaluated uncertainty surrounding vaccine characteristics and found that even if characteristics were changed, we would still see positive health impact and cost-effectiveness.
The decision for how to take these results forward to country-level introduction lies with the policy-maker, and how they are able to allocate their available budget.While we made some comparisons between products, the results of our study assume a reality where only one vaccine product is introduced.However, it is likely that both vaccine products could be introduced into the population, and the resulting health benefit could be increased.BCG is already licensed and recommended by the WHO for infants, and therefore BCG-revaccination of older adolescents and adults could be introduced earlier than M72/ AS01 E through a policy change.Resources may need to be spent on epidemiological studies investigating population characteristics, such as the infection prevalence, to determine where a vaccine effective in those who are uninfected will have the most impact.M72/AS01 E is still a vaccine candidate and forward progression depends on results from the phase III trial which has yet to start.More uncertainty surrounding costs and product characteristics exists, but overall M72/AS01 E predicted an increased health impact compared to BCG-revaccination.

Conclusions
We propose it is inadvisable to focus solely on one or two vaccine candidates to address the tuberculosis burden.While promising results have been seen from recent trials, it will be years before we can verify these characteristics, and therefore, we need a wide selection of options for the greatest likelihood of mitigating tuberculosis burden.We need to continue investment in all candidates currently in the pipeline, and support the development of new candidates, to increase the probability of success.
Our modelling suggests that M72/AS01 E and BCGrevaccination may substantially reduce the tuberculosis burden in India over future decades and would be costeffective regardless of the assumed product characteristics.We informed vaccine characteristics using clinical trial data but found variability in the vaccine profile as a crucial source of uncertainty.We cannot solely rely on M72/AS01 E and BCG-revaccination in case the realised characteristics differ considerably from expectations.Investment in multiple vaccine developments and delivery should be increased to raise the probability of success.
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Fig. 1
Fig. 1 Cumulative cases and deaths averted (in 1000 s) by 2050 from M72/AS01 E and BCG-revaccination scenarios.The top of the bar is the median estimate of the number averted for each scenario compared to the estimated number predicted by 2050 with the Status Quo no-new-vaccine baseline with 95% uncertainty range.The horizontal line is the median value of the Basecase for each vaccine.The cases and deaths averted by each scenario are compared to 72.2 (63.3-79.7) million incident tuberculosis cases and 13.8 (12.9-15.2) million tuberculosis deaths predicted by the Status Quo baseline between 2025 and 2050

Fig. 2
Fig. 2 Efficiency frontiers (discounted total costs [US$ billions] per disability-adjusted life year (DALY) averted) for Policy Scenarios for each vaccine product million between 2025 and 2050.The annual average cost-savings in treatment and diagnostics were US$60 (49-74) million over 2025-2050.The annual average cost of vaccination in the Basecase BCG-revaccination scenario was US$67 (29-122) million over 2025-2050.The annual average costsavings in treatment and diagnostics were US$43 (35-55) million over 2025-2050.The average annual cost of vaccination in the Basecase M72/AS01 E scenario was almost four times greater than the average annual cost of vaccination with the Basecase BCG-revaccination scenario.Accounting for cost-savings, the average annual incremental programme cost in the Basecase M72/AS01 E scenario (US$190 million) was over eight times greater than the average annual incremental programme cost with the Basecase BCG-revaccination scenario (US$23 million).

Fig. 3
Fig. 3 Comparison of ICERs for Vaccine Characteristic and Coverage Scenarios compared to the Status Quo no-new-vaccine baseline for each vaccine product.The Basecase M72/AS01 E scenario assumes a 50% efficacy POD vaccine efficacious with any infection status at the time of vaccination, with 10 years' duration of protection reaching 80% coverage for 15-year-olds and 70% coverage for those aged 16-34.Each M72/AS01 E scenario is delivered routinely to those aged 15 and as a campaign for those aged 16-34.The Basecase BCG-revaccination scenario assumes a 45% efficacy POI vaccine efficacious with no current infection at the time of vaccination, with 10 years duration of protection and reaching 80% coverage.Each BCG-revaccination scenario is delivered routinely to those aged 10 and as a campaign for those aged 11-18.The scenarios on the figure are labelled with the difference in product characteristics for that scenario compared to the Basecase.The 20 years' protection and 60% efficacy scenarios for M72/AS01 E overlap and appear as one point on the figure

Fig. 4
Fig. 4 Incremental costs by year until 2050 for the Basecase M72/AS01 E and BCG-revaccination scenarios compared to the Status Quo no-new-vaccine baseline.USD$, United States dollars

Table 1
Assumed M72/AS01 E and BCG-revaccination scenarios Abbreviations: AI Any infection; CI Current infection; NCI No current infection

Table 2
Cost-effectiveness analysis for M72/AS01 E and BCG-revaccination Policy ScenariosAbbreviations: DALYs Disability-adjusted life years; USD United States dollars