Gut microbiota in children with type 1 diabetes differs from that in healthy children: a case-control study

Table 1 Primers used for real-time PCR

Target group	Oligonucleotide sequence (5′-3′)	Reference	Amplicon size (bp)
Bacteroidetes	CATGTGGTTTAATTCGATGAT	Guo et al. 2008 [30]	126
	AGCTGACGACAACCATGCAG
Bacteroides	GAGAGGAAGGTCCCCCAC	Guo et al. 2008 [30]	106
	CGCTACTTGGCTGGTTCAG
Lactobacillus	GAGGCAGCAGTAGGGAATCTTC	Delroisse et al. 2008 [31]	126
	GGCCAGTTACTACCTCTATCCTTCTTC
Fusobacterium	CCCTTCAGTGCCGCAGT	Friswell et al. 2010 [32]	273
	GTCGCAGGATGTCAAGAC
Firmicutes	ATGTGGTTTAATTCGAAGCA	Guo et al. 2008 [30]	126
	AGCTGACGACAACCATGCAC
Actinobacteria	CGCGGCCTATCAGCTTGTTG	Stach et al. 2003 [33]	600
	CCGTACTCCCCAGGCGGGG
Bifidobacterium	CTCCTGGAAACGGGTGG	Matsuki et al. 2004 [34]	550
	GGTGTTCTTCCCGATATCTACA
Prevotella	GGTTCTGAGAGGAAGGTCCCC	Bekele et al. 2010 [35]	121
	TCCTGCACGCTACTTGGCTG
Enterococcus	CCCTTATTGTTAGTTGCCATCATT	Rinttilä et al. 2004 [36]	144
	ACTCGTTCTTCCCATGT
Proteobacteria	CATGACGTTACCCGCAGAAGAAG	Friswell et al. 2010 [32]	195
	CTCTACGAGACTCAAGCTTGC
ClostridiumCluster IV	GCACAAGCAGTGGAGT	Matsuki et al. 2004 [34]	239
	CTTCCTCCGTTTTGTCAA
Blautia coccoides-Eubacterium rectale group	CGGTACCTGACTAAGAAGC	Rinttilä et al. 2004 [36]	429
	AGTTTCATTCTTGCGAACG
Veillonella	ACCAACCTGCCCTTCAGA	Rinttilä et al. 2004 [36]	343
	CGTCCCGATTAACAGAGCTT
β-globin	GAAGAGCCAAGGACAGGTAC	Fredricks et al. 2007 [37]	270
	CAACTTCATCCACGTTCACC

ISSN: 1741-7015