Fig. 5

Hepatocyte-specific THBS1 knockout protects against D-gal/LPS-mediated liver failure in mice. A Schematic diagram representing ALF model establishment. B Kaplan–Meier survival analysis of mice during the ALF period. The survival of WT and hepatocyte-specific THBS1^KO mice was observed for 48 h., n = 18/group. C-E Temporal changes in serum ALT, AST and TBil levels. Data are represented as the mean ± SD, n = 10 for each time point. **** P < 0.0001 vs. WT. F Expression profile of Thbs1 in liver tissues. Data are represented as the mean ± SD, n = 10 for each time point. *** P < 0.001 vs. WT; Gapdh was used as a reference control gene. G Representative histopathological analysis, in which WT mice showed inflammatory cells and inflammatory infiltration (yellow arrow), massive bleeding and injury (purple arrow), hepatocyte death and hepatocellular structural loss (white arrow), and H THBS1 immunohistochemistry in liver tissues (black arrow) collected from mice at the normal control and D-gal/LPS-treated WT and hepatocyte-specific THBS1^KO mice