Fig. 4

Reduction of GAP43 and SNAP25 levels in the hippocampus of 5×FAD mouse brain. A-D Gap43 (A), Snap25 (B), neurogranin (C), and synaptotagmin 1(D) mRNA levels quantified using RT-qPCR. E–I Representative Western blots (E) and quantitative assessment (F–I) of GAP43 (E and F), SNAP25 (E and G), neurogranin (E and H), and synaptotagmin 1 (E and I) protein levels. Group comparison was performed using t-test. GAP43, growth-associated protein 43; Ng, neurogranin; mRNA, messenger RNA; RT-qPCR, real-time quantitative reverse transcription-polymerase chain reaction; SNAP25, synaptosome-associated protein 25; Syn 1, synaptotagmin 1; WT, wild-type