Fig. 5

Construction of a neural-cell-targeting EV system loaded with mRNAs of Gap43 and Snap25. A Schematic representation of generation, collection, and administration of neural-cell-targeting EVs overexpressing Gap43 and Snap25 mRNAs. B HA pulldown assays and representative Western blots of LAMP-2B in HEK293T cells transfected with HA-RVG-LAMP-2B plasmid and their corresponding EVs. C RT-qPCR of HEK293T cells transfected with HA-RVG-LAMP-2B detects the mRNA of Rvg. D–G Quantitative assessment of Gap43 (D and F) and Snap25 (E and G) in HEK293T cells (D and E) and EVs (F and G) using RT-qPCR. H HA pulldown assays and representative Western blots of 5×FAD hippocampal tissues treated with EVs-TNGS. n = 3. I–K Representative Western blots (I) and their quantitative assessment (J and K) of protein levels of GAP43 (I and J) and SNAP25 (I and K) in treated 5×FAD mouse brains. EVs, extracellular vesicles; EVs-TN, extracellular vesicles targeting neural cells; EVs-TNGS, extracellular vesicles targeting neural cells overexpressing Gap43 and Snap25; GAP43, growth-associated protein 43; mRNAs, messenger RNAs; SNAP25, synaptosome-associated protein 25; RT-qPCR, real-time quantitative reverse transcription-polymerase chain reaction; RVG, rabies virus glycoprotein