Figure 12

Immunohistochemistry results of transplanted ALF pig liver samples. (A) No ALB⁺cells were found in liver puncture biopsy samples 8 weeks after transplantation in Group IV (40×); (B) ALB⁺ cells were detected 6 weeks after hPMSCs infusion in the specimen harvested using an automatic biopsy needle (40×); (C-H) ALB⁺cells were observed in three pigs at 5 months (C, E, G, 20×; D, F, H, 40×); (I-P) CK 18⁺cells were observed in three pigs at 5 months (I, K, M, O, 20×; J, L, N, P, 40×); (Q, S) non-transplanted pig livers served as negative controls for ALB (Q) and CK 18 (S) staining (20×); (R, T) sections of human liver served as positive controls for ALB (R) and CK 18 (T) staining (40×). It is noteworthy that almost all ALB⁺ or CK18⁺cells were adjacent to vascular structures.