Figure 13

Detection of human-specific hepatic markers by RT-PCR (A) and alu repeat sequences by PCR (B). (A): Lane 1, positive control (human liver for ALB and CK 18, hepatocellular carcinoma liver for AFP; lane 2: Group I as a negative control; lane 3 to 10, pig livers transplanted after 4, 6, 8, 12, 16, and 20 weeks and 5 months (double). Before 5 months, the samples were all obtained by liver biopsy puncture. Human ALB and CK18 were detected in the sixth week (lane 4) and the fifth month (lanes 9 and 10). (B): Lane 1, positive control (human DNA); lanes 2 to 6, Group IV (2, 4, 7, and 156 days after cell transplantation); lanes 7 and 8, Group III (2 and 4 days after cell transplantation); lanes 9 and 10, Group II (4 and 24 days after cell transplantation); lane 11, Group I (4 days after GalN infusion).