Figure 3

Adipogenic (A-C, H) and osteogenic (D-G, I) differentiation of hPMSCs. (A) hPMSCs maintained in normal growth medium (10×); (B) human adipose tissue as a positive control (10×); (C) hPMSCs that had undergone adipogenic differentiation (accumulation of lipid droplets shown by red staining) (20×); (D) hPMSCs maintained in normal growth medium; (E) osteoblast as a positive control(4×); (F, G) hPMSCs that had undergone osteogenic differentiation (bone nodules formed), before and after staining (4×). (H, I) Representative RT-PCR analyses of hPMSCs cultured in adipogenic and osteogenic medium for 28 days. No LPL expression was found in hPMSCs maintained in regular medium (H, lane 2). After adipogenic induction, hPMSCs expressed LPL mRNA (H, lane 1). Human adipose tissue expressed LPL mRNA (H, lane 3). No OPN expression was found in hPMSCs maintained in growth medium (I, lane 2). After osteogenic induction, hPMSCs expressed OPN mRNA (I, lane 1). Osteoblasts expressed OPN mRNA (I, lane 3). LPL, lipoprotein lipase; OPN, osteopontin.