Influence of the ALDH2 agonist Alda-1 on high glucose-induced responses of aconitase activity and cardiomyocyte contractile properties. Cardiomyocytes from control FVB mice were exposed to high glucose (HG; 30 mM) in the absence or presence of Alda-1 (20 μM), the mitochondrial uncoupler FCCP (1 μM) or the GSK3β inhibitor SB216763 (10 μM) for 12 hours prior to assessment of mitochondrial and mechanical properties. (A) Aconitase activity; (B) peak shortening (PS; normalized to cell length); (C) maximal velocity of shortening (+ dL/dt); (D) maximal velocity of relengthening (- dL/dt); (E) time-to PS (TPS); (F) time-to-90% relengthening (TR90). Mean ± SEM, n = 5 isolations (panel A) or 72 to 73 cells per group (Panel B-F). *P < 0.05 versus control group; #
P < 0.05 versus HG group.