MitoQ suppresses caspase-1-mediated IL-1 beta and IL-18 release during colitis. ELISA assay for (A) IL-1 beta or (B) IL-18 were performed in colon homogenates. Results are expressed as mean ±SE. n=5. *P <0.001,**P<0.01. (C) mRNA expression levels of IL-1 beta and IL-18 in colon tissue were examined by RT-PCR. (D) Cleavages of caspase-1, IL-1 beta and IL-18 were analyzed by western blotting analysis in colon homogenates.