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Table 1 Oligonucleotide primers used for quantitative RT-PCR

From: A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol

Target gene Primer sequence Length of amplicon GenBank accession no.
Abcg5 Forward: 5'-AGGTCATGATGCTAGATGAGC-3' 260 bp AH011511
  Reverse: 5'-CAAAGGGATTGGAATGTTCAG-3'   
Abcg8 Forward: 5'-CTCCTCGGAAAGTGACAACAG-3' 190 bp AH011518
  Reverse: 5'-TAGATTTCGGATGCCCAGCTC-3'   
Hmgr Forward: 5'-CCGGCAACAACAAGATCTGTG-3' 114 bp BB664708
  Reverse: 5'-ATGTACAGGATGGCGATGCA-3'   
Cyp7a1 Forward: 5'-CAGGGAGATGCTCTGTGTTCA-3' 121 bp NM_007824
  Reverse: 5'-AGGCATACATCCCTTCCGTGA-3'   
Abca1 Forward: 5'-CCCAGAGCAAAAAGCGACTC-3' 89 bp NM_013454
  Reverse: 5'-GGTCATCATCACTTTGGTCCTTG-3'   
Mdr2 (Abcb4) Forward: 5'-GCAGCGAGAAACGGAACAG-3' 64 bp NM_008830
  Reverse: 5'-GGTTGCTGATGCTGCCTAGTT-3'   
LXRα Forward: 5'-GCTCTGCTCATTGCCATCAG-3' 79 bp AF085745
  Reverse: 5'-TGTTGCAGCCTCTCTACTTGGA-3'   
Srebp-1c Forward: 5'-GGAGCCATGGATTGCACATT-3' 103 bp BI656094
  Reverse: 5'-CCTGTCTCACCCCCAGCATA-3'   
Srebp-2 Forward: 5'-CTGCAGCCTCAAGTGCAAAG-3' 119 bp AF374267
  Reverse: 5'-CAGTGTGCCATTGGCTGTCT-3'   
Cyclophilin Forward: 5'-AAGTTCCATCGTGTCATCAAGGAC-3' 173 bp M60456
  Reverse: 5'-CCATTGGTGTCTTTGCCTGC-3'