VHL regulation of miRNA expression in RCC4 cells. Expression of VHL-regulated miRNAs was validated by relative quantitation real-time PCR (qPCR). (a) VHL-dependent downregulation of miR-31, miR-21 and let-7i in RCC4-VHL cells (P = 0.05). (b) VHL-dependent upregulation of miR-155, miR-210, miR-193b, miR-17, miR-18a and miR-20a in RCC4-VHL (P = 0.05). The expression of the miRNAs was normalised to RNU6B expression. The mean ± SEM of three independent cell cultures is shown, and each sample was assayed in triplicate.