miRNA expression in RCC4-VHL cells following hypoxia-inducible factor (HIF) small interfering RNA (siRNA) treatment. Expression of VHL-regulated miRNAs in RCC4-VHL cells in response to HIF-1α and HIF-2α siRNA treatment was assessed by qPCR. (a) Expression of miR-210 is decreased in response to HIF siRNA treatment in RCC4-VHL cells (P = 0.05). (b) Expression of miR-155 is also decreased upon treatment with HIF siRNA in RCC4-VHL cells (P = 0.05). The expression of the miRNAs was normalised to RNU6B. The mean ± SEM of three independent cell cultures is shown, and each sample was assayed in triplicate.