Western blot gel (a) and histogram (b) showing relative expression of Fas, FasL and active caspase-3 proteins in the spleen. (a) Western blot showed that the expression of Fas, FasL and active caspase-3 were upregulated with the increasing concentrations of atrazine (2-chloro-4-ethytlamino-6-isopropylamine-1,3,5-triazine; ATR) (0, 100, 200 and 400 mg/kg). β Actin was used as the internal control. (b) Quantification analysis of the western blot. The results were expressed as the ratio of target protein/β actin in each group. *P <0.05, compared to control group.