Akt phosphorylation in vivo. Akt phosphorylation plays a central role in a variety of oncogenic processes. Compared to the number of cells positive for phospho-Akt-Thr308 in the mammary carcinoma tissue of a control mouse (A), the number of phospho-Akt-Thr308-positive cells was markedly lower in the mammary carcinoma tissue of a mouse treated with 20 mg/kg/day α-mangostin (B). Note that stronger nuclear and weaker cytoplasmic staining was observed in both a control mouse carcinoma (C, higher magnification of the box in A) and a 20 mg/kg/day α-mangostin-treated mouse carcinoma (D, higher magnification of a box in B). The numbers and intensities of stained cells were weaker in the 20 mg/kg/day group ( B, D) than in the control group (A, C). (A, B): Scale bar = 100 μm; (C, D): Scale bar = 25 μm. (A-D): Phospho-Akt-Thr308 immunohistochemistry.