Expression profile of miR-200a, miR-200c, miR-203, and miR-205 in lymph node samples containing macrometastases (Ma; n = 18), micrometastases/isolated tumor cells (Mi and isolated tumor cells; n = 7), or in non-metastatic lymph node specimens (NM; n = 13). The Y-axis shows the log10 fold-change of the relative expression (2-ΔΔCt). The P value (Mann–Whitney) from each comparison is provided. The dotted line indicates the cutoff adopted according to the Youden index (value in which the difference between sensitivity and 1-specificity is maximum) obtained from the ROC curves analysis. The horizontal line indicated the median of fold-change values for each group.