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Table 2 Sensitivity and specificity values of microRNAs evaluated in discriminating metastatic and non-metastatic lymph nodes in FFPE and FNA biopsies from lymph node samples

From: Accuracy of microRNAs as markers for the detection of neck lymph node metastases in patients with head and neck squamous cell carcinoma

microRNA   Sensitivity Specificity
Cutoffa Metastaticb Macrometastases Micrometastases Isolated tumor cells Non-metastatic
% (95% CI) (n) % (95% CI) (n) % (95% CI) (n) % (95% CI) (n) % (95% CI) (n)
FFPE samples
miR-200a 5.96 76.0 (54.5–89.8) (19/25) 94.4 (70.6–99.7) (17/18) 40.0 (7.3–82.9) (2/5) 0 (0–80.2) (0/2) 100 (71.7–100) (13/13)
miR-200c 2.33 88.0 (67.7–96.8) (22/25) 100 (78.1–100) (17/18) 80.0 (29.9–98.9) (4/5) 0 (0–80.2) (0/2) 100 (71.7–100)) (13/13)
miR-203 1.96 100 (83.4–100) (25/25) 100 (78.1–100) (17/18) 100 (46.3–100) (5/5) 100 (19.8–100) (2/2) 100 (71.7–100) (13/13)
miR-205 1.54 100 (83.4–100) (25/25) 100 (78.1–100) (17/18) 100 (46.3–100) (5/5) 100 (19.8–100) (2/2) 100 (71.7–100) (13/13)
FNA samples classified by cytology
miR-203 10 100 (91.5–100) (42/42) 100 (91.5–100) (42/42) N/A N/A 100 (94.9–100) (71/71)
miR-205 10 100 (91.5–100) (42/42) 100 (91.5–100) (42/42) N/A N/A 100 (94.9–100) (71/71)
FNA samples classified by histology
miR-203 10 92.9 (80.5–98.4) (68/71) 100 (89.3–100) (68/68) 0 (0) (0/2) 0 (0) (0/1) 100 (94.9–100) (71/71)
miR-205 10 92.9 (80.5–98.4) (68/71) 100 (89.3–100) (68/68) 0 (0) (0/2) 0 (0) (0/1) 100 (94.9–100) (71/71)
  1. FFPE, Formalin-fixed paraffin embedded; FNA, Fine-needle aspiration; PPV, Positive predictive value; NPV, Negative predictive value; AUC, Area under the ROC curve; CI, Confidence interval; N/A, Not applicable. aThe cutoff values for FFPE samples were determined according to the Youden index (value in which the difference between sensitivity and 1-specificity is maximum) obtained from the ROC curves. bThe ‘metastatic’ group comprises all cases with positive lymph nodes (macrometastases, micrometastases, or isolated tumor cells).