Distribution of antibody titers to individual merozoite antigens among the three groups of children. The panels show the distribution of antibody titers (median and interquartile range) in the malaria-free (blue circles), single-episode (green triangles) and multiple-episodes (red squares) groups of children at six cross-sectional surveys for the respective antigens: A) MSP-119, B) MSP-2_Dd2, C) MSP-2_CH150/9, D) MSP-3_3D7, E) AMA-1_FVO, F) AMA-1_3D7 and G)PfRh2. ‘NC’ refers to antibody titers in sera from P. falciparum-naïve adults (used here as negative controls). ‘PHIS’ refers to antibody titers in a pool of hyperimmune sera (used here as a positive control). The black bold dotted line shows the ‘threshold’ antibody concentrations to respective antigens that were calculated as described in the Results section. The thin dotted blue line shows the ‘seropositivity cut off’ based on the mean plus two standard deviations of the antibody titer obtained with the negative control sera.