Fig. 4

Cell viability assayed by mitochondrial metabolism assessment (MTT test). NSC-34 neuron-like cells were incubated with different concentrations of mfNDs, alum and AluDia particles for 72 hours. AluDia concentrations tested in (c) = mfNDs concentrations in (a) + alum concentrations in (b). a: mfNDs particles are non-toxic, except at the highest dose. b: alum particles display a toxic or severely toxic effect at all doses used. c: AluDia particles have no supplemental toxicity compared to alum alone. Viability was normalized to the value determined in untreated cells. Results are expressed as mean ± standard deviation. Cells were obtained from four different cultures to realize four biological replications (n = 4). Viability measurement of each concentration point was repeated 12 times. *Significant difference at p < 0.05. AluDia Alhydrogel^® and mfND complex, mfNDs modified fluorescent nanodiamonds