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Table 2 Intra-adrenal steroids measured by LC/MS

From: The human fetal adrenal produces cortisol but no detectable aldosterone throughout the second trimester

    Total Control female Smoke-exposed female Control male Smoke-exposed male
Steroid LOQ (ng/mL MeOH) LOQ (adjusted)a QSb (n = 60) Mean ± SEM (ng/mg tissue) QS (n = 15) Mean ± SEM (ng/mg tissue) QS (n = 15) Mean ± SEM (ng/mg tissue) QS (n = 15) Mean ± SEM (ng/mg tissue) QS (n = 15) Mean ± SEM (ng/mg tissue)
Pregnenolone 10 0.05 58 5.019 ± 0.49 15 4.619 ± 0.79 13 4.299 ± 0.927 15 4.073 ± 0.828 15 7.087 ± 1.135
17α-Hydroxyprogesterone 1 0.005 60 1.291 ± 0.182 15 1.086 ± 0.151 15 1.115 ± 0.189 15 0.878 ± 0.159 15 2.087 ± 0.624
DHEAS 0.1 0.0005 60 1.153 ± 0.178 15 1.426 ± 0.412 15 0.782 ± 0.16 15 0.966 ± 0.271 15 1.437 ± 0.468
Progesterone 1 0.005 60 1.089 ± 0.185 15 0.993 ± 0.194 15 0.95 ± 0.278 15 1.066 ± 0.504 15 1.347 ± 0.415
Cortisol 0.1 0.0005 56 0.626 ± 0.087 14 0.713 ± 0.198 14 0.498 ± 0.127 13 0.471 ± 0.142 15 0.824 ± 0.198
Corticosterone 0.1 0.0005 57 0.354 ± 0.075 13 0.265 ± 0.073 14 0.213 ± 0.08 15 0.345 ± 0.153 15 0.593 ± 0.224
16α-Hydroxyprogesterone 0.1 0.0005 59 0.259 ± 0.035 15 0.259 ± 0.036 14 0.18 ± 0.04 15 0.192 ± 0.038 15 0.407 ± 0.116
11-Deoxycortisol 0.1 0.0005 60 0.119 ± 0.017 15 0.148 ± 0.039 15 0.1 ± 0.03 15 0.082 ± 0.02 15 0.146 ± 0.042
Cortisone 1 0.005 37 0.108 ± 0.018 9 0.15 ± 0.047 9 0.096 ± 0.034 9 0.075 ± 0.023 10 0.109 ± 0.037
Testosterone 0.1 0.0005 32 0.032 ± 0.007 9 0.062 ± 0.02 8 0.028 ± 0.013 7 0.017 ± 0.008 8 0.023 ± 0.008
Deoxycorticosterone 0.1 0.0005 39 0.023 ± 0.003 12 0.03 ± 0.006 10 0.018 ± 0.003 10 0.019 ± 0.004 7 0.025 ± 0.009
Corticosterone sulphate 10 0.05 10 0.018 ± 0.01 3 0.022 ± 0.019 2 0.003 ± 0.002 1 0.001 ± 0.001 4 0.045 ± 0.032
11-Dehydrocorticosterone 0.1 0.0005 12 0.003 ± 0.001 4 0.005 ± 0.004 3 0.001 ± 0 2 0.002 ± 0.002 3 0.005 ± 0.003
Aldosterone 1 0.005 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ
DHEA 10 0.05 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ
Δ4-Androstenedione 10 0.05 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ
Δ5-Androstenediol 1 0.005 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ
17α-Hydroxypregnenolone 100 0.5 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ 0 < LOQ
Cortisone sulphate 10 0.05 0 < LOQ 1 < LOQ 1 < LOQ 1 < LOQ 1 < LOQ
  1. aEquivalent to ng/mg of tissue LOQ
  2. bQuantifiable samples out of n samples as stated in brackets
  3. LOQ limit of quantitation, QS quorum sensing, SEM standard error of the mean