Fig. 4

High variability among RTS,S vaccine-induced IgG targeting the central repeat and C-terminal regions of CSP. Children in RTS,S vaccine group from Manhiça and Ilha Josina cohorts (N = 99, 3 M) were tested for IgG to NANP and CT and C1q-fixation to CSP, and the values were used for the following analysis. a Heat map of children arranged in descending (top to bottom) order of C1q-fixation (left), corresponding IgG to NANP and CT (middle), and for comparison IgM to NANP and CT (right). b Variability between epitope specificity was quantified by calculating the ratio of NANP-to-CT IgG. Children with low variability were considered to have equal reactivity to NANP and CT (ratio between 0.75 and 1.25 shown in white), and children exceeding this range were considered to have a NANP- or CT-skewed response (ratio > 1.25 shown in purple and ratio < 0.75 shown in blue, respectively). Children were arranged in descending order of C1q-fixation (left to right), whereby those in the top quantile are in black bars (C1q-high, OD range 2.03 to 4.00) and those in the bottom quantile are in gray bars (C1q-low, OD range 0.02 to 1.97). c Pie charts representing the percentage of children with the following epitope-specific IgG profiles from C1q-high and C1q-low groups: NANP-skewed, equal, and CT-skewed