Fig. 2
From: NG2 glia regulate brain innate immunity via TGF-β2/TGFBR2 axis
Olig1+ cell ablation augments neuroinflammation following LPS treatment. a Representative graph showing relative mRNA levels of inflammatory mediators in the indicated brain regions from DTROlig1 Tg and littermate control animals (n = 9–13 mice). b Representative western blots showing IL-1β expression in the indicated brain regions from adult mice with Olig1+ cell ablation. Olig1-Cre serve as negative control. Animals were sacrificed 4 h after LPS challenge. c Quantification of data shown in b (n = 5–6 mice). d Oligodendrocyte ablation does not significantly alter the levels of inflammatory mediators in the adult mouse brain following LPS challenge. qPCR analysis of the indicated brain regions including the hippocampus, substantia nigra (SN), striatum, and cerebrocortex 4 h after systemic LPS (2.5 mg kg−1, i.p.) administration. Data are expressed as mean ± SEM for all the data. Two-way ANOVA with Bonferroni’s post test was used for the statistical analysis (n = 3–8). No significance is found. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001