Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

Table 2 Comparison of the serum antibody levels of HDs versus those of patients with transient ischemic attack (TIA) or acute ischemic stroke (AIS)

Sample information		HD	TIA	AIS
Total sample number		285	92	464
Male/female		188/97	55/37	271/193
Age (average ± SD)		52.3 ± 11.7	70.2 ± 11.6	75.5 ± 11.5
Alpha analysis (antibody level)		DIDO1-Ab	FOXJ2-Ab	CPSF2-Ab
HD	Average	4736	8568	2515
	SD	3179	5103	1061
	Cutoff value	11,095	18,774	4638
	Positive no.	19	16	12
	Positive (%)	6.7%	5.6%	4.2%
TIA	Average	6950	12,390	3792
	SD	5251	7533	4280
	Positive no.	14	13	15
	Positive (%)	15.2%	14.1%	16.3%
	P (TIA vs HD)	0.0002	< 0.0001	0.0056
AIS	Average	7309	13,255	3291
	SD	5415	8042	2396
	Positive no.	81	91	69
	Positive (%)	17.5%	19.6%	14.9%
	P (AIS vs HD)	< 0.0001	< 0.0001	< 0.0001

The upper panel indicates the numbers of all samples and samples from males and females as well as the ages (average ± SD). The lower panel summarizes the serum antibody levels (alpha luminescent photon count) examined by AlphaLISA. Purified DIDO1 (amino acids 1-275)-glutathione S-transferase (GST) protein and synthetic peptides, bFOXJ2-426 and bCPSF2-607, were used as antigens. The cutoff values were determined as the average HD values plus two SDs, and positive samples for which the Alpha counts exceeded the cutoff value were scored. P values were calculated using the Kruskal–Wallis test. P values lower than 0.05 and positive rates higher than 10% are marked in bold. Box-whisker plots of the same results are shown in Fig. 2a, d, and g

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