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Table 2 Comparison of the serum antibody levels of HDs versus those of patients with transient ischemic attack (TIA) or acute ischemic stroke (AIS)

From: Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

Sample information HD TIA AIS
      Total sample number 285 92 464
      Male/female 188/97 55/37 271/193
      Age (average ± SD) 52.3 ± 11.7 70.2 ± 11.6 75.5 ± 11.5
Alpha analysis (antibody level) DIDO1-Ab FOXJ2-Ab CPSF2-Ab
HD Average 4736 8568 2515
SD 3179 5103 1061
Cutoff value 11,095 18,774 4638
Positive no. 19 16 12
Positive (%) 6.7% 5.6% 4.2%
TIA Average 6950 12,390 3792
SD 5251 7533 4280
Positive no. 14 13 15
Positive (%) 15.2% 14.1% 16.3%
P (TIA vs HD) 0.0002 < 0.0001 0.0056
AIS Average 7309 13,255 3291
SD 5415 8042 2396
Positive no. 81 91 69
Positive (%) 17.5% 19.6% 14.9%
P (AIS vs HD) < 0.0001 < 0.0001 < 0.0001
  1. The upper panel indicates the numbers of all samples and samples from males and females as well as the ages (average ± SD). The lower panel summarizes the serum antibody levels (alpha luminescent photon count) examined by AlphaLISA. Purified DIDO1 (amino acids 1-275)-glutathione S-transferase (GST) protein and synthetic peptides, bFOXJ2-426 and bCPSF2-607, were used as antigens. The cutoff values were determined as the average HD values plus two SDs, and positive samples for which the Alpha counts exceeded the cutoff value were scored. P values were calculated using the Kruskal–Wallis test. P values lower than 0.05 and positive rates higher than 10% are marked in bold. Box-whisker plots of the same results are shown in Fig. 2a, d, and g