Fig. 1

Antibodies from malaria-exposed PNG pregnant women mediated complement fixation on CS2 pRBCs. Complement A C1q and B C3 fixation on CS2 pRBCs by flow cytometry. Complement fixation was tested using antibodies from PNG pregnant women (n=302) and Australian malaria non-exposed donors (n=15) in the presence of complement (NS). C Complement C3 fixation on CS2 pRBCs in the presence of NS or HIS by flow cytometry. CS2 pRBCs were opsonised with antibodies from 35 randomly selected PNG pregnant women or Australian malaria non-exposed donors (N=13) and in the presence of either normal serum or heat inactivated serum. Complement fixation is presented as the average geometric mean fluorescence intensity (gMFI) of each sample tested in duplicate and the red line represents the median gMFI for all samples tested. D C1q and C3 fixation by Western blot using magnet purified CS2 pRBCs that were first opsonized with a pool of PNG or malaria non-exposed antibodies, in the presence of NS. Unopsonized sample (CS2 pRBCs incubated without antibody, but with NS) was used as a negative control and heat shock protein 70 (HSP70) as a loading control