Fig. 4

Immunohistochemical staining of renal neoplasms with four markers identified by TPA. A Staining of perilipin 2 (PLIN2), beta-tubulin III (TUBB3), lysosomal associated membrane protein 1 (LAMP1), and hexokinase 1 (HK1) in renal neoplasm subtypes. B Bar graphs representing the number of cases and extent of staining for PLIN2, TUBB3, LAMP1, and HK1. For PLIN2 and TUBB3, the percentage of tumor cells that stained positive was scored as 0 for negative, 1 for 1–10%, 2 for > 10–50%, and 3 for > 50%. For HK1, the extent of staining was scored as 0 for negative, 1 for focal or few cells, 2 for moderate, and 3 for abundant. Subtype abbreviations: ccRCC, clear cell renal cell carcinoma; pRCC, papillary renal cell carcinoma; chRCC, chromophobe renal cell carcinoma; RO, renal oncocytoma