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Table 1 Antibodies, dilutions, and retrieval buffers used

From: The effects of selected inhibitors on human fetal adrenal steroidogenesis differs under basal and ACTH-stimulated conditions

Antibody

Dilution

Retrieval buffer

Species

Supplier

Number

BrdU

1:100

CIT

Mouse

Dako

M0744

cPARP

1:500

CIT

Rabbit

Cell Signaling

5625

CYP17A1

1:1,500

CIT

Rabbit

Abcam

Ab134910

CYP11B2*

1:200

TEG

Rabbit

Sigma

HPA049171

CYP21A2

1:15,000

TEG

Goat

Santa Cruz

Sc-48466

  1. TEG buffer: 10 mM Tris, 0.5 mM EGTA, pH 9.0; citrate (CIT) buffer: 10 mM, pH 6.0. *The antigen sequence is 96% identical with CYP11B1
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BMC Medicine

ISSN: 1741-7015

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