Fig. 3

The 5mC clusters and 5mC score correlated with immune phenotypes in the TCGA-BLCA cohort. A The differences in cancer immunity cycles between 5mC clusters. B, C The differences in infiltration levels of TIICs between 5mC clusters in the TIMER and MCP-counter algorithms. The asterisks indicate a statistically significant p value calculated using the Mann-Whitney U or t-test (*P < 0.05; **P < 0.01; ***P < 0.001). D The lower left part indicates the correlations between the 5mC score and cancer immunity cycles; the upper right part shows the correlations between the 5mC score and effector genes of several anticancer TIICs, including CD8+ T cells, NK cells, macrophages, Th1 cells, and dendritic cells. E The correlation between the 5mC score and the infiltration levels of five anticancer TIICs (CD8+ T cells, NK cells, macrophages, Th1 cells, and dendritic cells), which were calculated using seven independent algorithms.