Fig. 5

TP deficiency increases ROS levels and mitochondrial respiration in ASC cells. Data were obtained at D0 in undifferentiated ASC, ASC with a CRISPR-Cas9-mediated TP-knockout (KO), and ASC with a Cas9/scramble gRNA KO corresponding to control (CTL) cells. Differences between the three cell lines were determined by analysis of variance (ANOVA) with Bonferroni’s post hoc multiple comparison test. All results are expressed as means ± SEM of five independent experiments. A Reactive oxygen species (ROS) production assessed by oxidation of 5-6-chloromethyl-2,7-dichlorodihydro-fluorescein diacetate (CM-H₂DCFDA) in ASC. Results were normalized to DNA content measured by DAPI. ****p < 0.0001. B The respiratory flux profiles of cells were determined using a Seahorse Bioanalyzer as described in the “Methods” section. Data are from six replicates and are a compilation of three independent experiments. C Quantification of bio-energetic parameters including oxygen consumption rate (OCR) associated to basal respiration, ATP-linked respiration, proton leak, and maximal respiration capacity. *p < 0. 05, **p < 0. 01, n.s., non-significant