Fig. 1

Representative flow cytometry gating strategy for identification of SARS-CoV-2 reactive CD4⁺ and CD8⁺ T cells. Representative dot plots illustrate gating strategy for identification of lymphocytes according to FSC-A and SSC-A (A), single-cell gating using FSC-A and FCS-H (B), and the gating of CD3⁺ cells according to the expression of CD3 (C). The CD3⁺ cell population was used to gate CD4⁺ and CD8⁺ T cell populations (D), and finally reactive antigen-specific cells were gated according to the co-expression of CD137 and CD69 in the CD4⁺ (E) or CD8⁺ (F) populations