Fig. 1
From: Efficacy of SCF drug conjugate targeting c-KIT in gastrointestinal stromal tumor
Expression, purification, and bioactivity evaluation of rhSCF. A Immunoblotting analysis of purified rhSCF protein. Lane 1: pre-stained protein marker; lanes 2 and 4: reference rhSCF from Sf9 cells. Due to glycosylation modification, rhSCF from Sf9 cells has a higher molecule weight than rhSCF without glycosylation; lanes 3 and 5: purified rhSCF. Left panel: PVDF membrane captured by using stain free method on Bio-Rad ChemiDoc system. Right panel: western blot result of rhSCF reference and purified rhSCF. B SDS-PAGE result of rhSCF after purification. Lane 1: protein marker; lane 2: sample after Ni–NTA Sepharose purification; lane 3: sample after phenyl Sepharose purification. C Immunoblot analysis of phosphorylation status of KIT signaling proteins in THP-1 treated by rhSCF (100 ng/mL). D, E Membrane KIT expression levels in THP-1 (D) and GIST 430 (E) treated by rhSCF (100 ng/mL). The vertical dot lines in D and E were given to view the induction effect of SCF. Numbers indicated left-side or right-side cell population (%) separated by dot lines in each group